GPR34 Protein

Catalog No. ABIN7538271

Product Details

Target: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human GPR34 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Alternative Name: GPR34 (GPR34 Products)

Synonyms: GPR34 Protein, fl10e05 Protein, wu:fl10e05 Protein, zgc:110315 Protein, G protein-coupled receptor 34 Protein, G protein-coupled receptor 34a Protein, G protein-coupled receptor 34b Protein, GPR34 Protein, Gpr34 Protein, gpr34a Protein, gpr34b Protein

Background: G protein-coupled receptors (GPCRs), such as GPR34, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response.[supplied by OMIM, Apr 2006]

Molecular Weight: The human full length GPR34 protein has a MW of 43.9kDa

UniProt: Q9UPC5