GPR25 Protein
GPR25
Origin: Human
Host: Mammalian Cells
Synthetic Nanodisc
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- Target See all GPR25 products
- GPR25 (G Protein-Coupled Receptor 25 (GPR25))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- Mammalian Cells
- Purpose
- Human GPR25 full length protein-synthetic nanodisc
- Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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- Comment
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Advantages of Synthetic Nanodiscs:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- GPR25 (G Protein-Coupled Receptor 25 (GPR25))
- Alternative Name
- GPR25 (GPR25 Products)
- Synonyms
- Gm1300 Protein, G protein-coupled receptor 25 Protein, GPR25 Protein, Gpr25 Protein
- Background
- This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]
- Molecular Weight
- The human full length GPR25 protein has a MW of 38.8kDa
- UniProt
- O00155
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