CRHR2 Protein

Catalog No. ABIN7538209

Product Details

Target: CRHR2 (Corticotropin Releasing Hormone Receptor 2 (CRHR2))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human CRFR2 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CRHR2 (Corticotropin Releasing Hormone Receptor 2 (CRHR2))

Alternative Name: CRFR2 (CRHR2 Products)

Synonyms: CRF-RB Protein, CRF2 Protein, CRFR2 Protein, HM-CRF Protein, CRF-R2 Protein, CRFR2alpha Protein, CRFR2beta Protein, Crfr2 Protein, Crf2r Protein, CRH-R2 Protein, crhr2-A Protein, corticotropin releasing hormone receptor 2 Protein, corticotropin releasing hormone receptor 2 S homeolog Protein, CRHR2 Protein, Crhr2 Protein, crhr2.S Protein

Background: The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]

Molecular Weight: The human full length CRFR2 protein has a MW of 47.7kDa

UniProt: Q13324

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Feeding Behaviour