ADRB3 Protein

Catalog No. ABIN7538128

Product Details

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human ADRB3 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Alternative Name: ADRB3 (ADRB3 Products)

Synonyms: BETA3AR Protein, Adrb-3 Protein, ADRB Protein, BAR3 Protein, B3AR Protein, GPCR Protein, im:7154935 Protein, si:ch211-215f19.1 Protein, zgc:194843 Protein, zgc:194852 Protein, adrenoceptor beta 3 Protein, adrenergic receptor, beta 3 Protein, adrenoceptor beta 3a Protein, adrenoceptor beta 3b Protein, ADRB3 Protein, Adrb3 Protein, adrb3a Protein, adrb3b Protein

Background: The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.[provided by RefSeq, Oct 2019]

Molecular Weight: The human full length ADRB3 protein has a MW of 43.5kDa

UniProt: P13945

Pathways: cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation