ADRB1 Protein

Catalog No. ABIN7538126

Product Details

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: Mammalian Cells

Purpose: Human ADRB1 full length protein-synthetic nanodisc

Characteristics: Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.

Application Details

Comment:

Advantages of Synthetic Nanodiscs:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins

Limitations of Synthetic Nanodiscs:

• Intolerant to acids and high concentrations of divalent metal ions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Alternative Name: ADRB1 (ADRB1 Products)

Synonyms: ADRB1R Protein, B1AR Protein, BETA1AR Protein, RHR Protein, RATB1AR Protein, X-Beta1AR Protein, BAR1 Protein, zgc:194728 Protein, zgc:194731 Protein, ADRB1 Protein, Adrb-1 Protein, beta-AR Protein, adrenoceptor beta 1 Protein, adrenoceptor beta 1 L homeolog Protein, adrenergic, beta-1-, receptor Protein, adrenergic receptor, beta 1 Protein, ADRB1 Protein, Adrb1 Protein, adrb1.L Protein, adrb1 Protein

Background: The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Beta-1 adrenoceptors are predominately located in the heart. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure.

Molecular Weight: The human full length ADRB1 protein has a MW of 51.2 kDa

UniProt: P08588

Pathways: cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation