FCGR2B Protein (AA 46-217) (His-Avi Tag,Biotin)

Catalog No. ABIN7536870

Product Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Protein Type: Recombinant

Protein Characteristics: AA 46-217

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR2B protein is labelled with His-Avi Tag,Biotin.

Application: SDS-PAGE (SDS), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC), Surface Plasmon Resonance (SPR)

Purpose: Biotinylated human Fc gamma RIIb / CD32b protein

Sequence: APPKAVLKLE PQWINVLQED SVTLTCRGTH SPESDSIQWF HNGNLIPTHT QPSYRFKANN NDSGEYTCQT GQTSLSDPVH LTVLSEWLVL QTPHLEFQEG ETIVLRCHSW KDKPLVKVTF FQNGKSKKFS RSDPNFSIPQ ANHSHSGDYH CTGNIGYTLY SSKPVTITVQ APGGGLNDIF EAQKIEWHEG GGENLYFQSG GHHHHHHHHH H

Specificity: IgG

Characteristics: The sequence of the extracellular domain of human CD32b (Ala 46-Pro 217) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Purification: Nickel and SEC

Purity: > 95 % by SEC-HPLC

Endotoxin Level: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIb / CD32b protein, immobilized on a CM5 chip via an anti-His antibody, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 15 μM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Concentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Preservative: Without preservative

Storage: RT,4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Expiry Date: 12 months

Target Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: CD32b (FCGR2B Products)

Synonyms: CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc fragment of IgG, low affinity IIb, receptor (CD32) Protein, Fc receptor, IgG, low affinity IIb Protein, low affinity immunoglobulin gamma Fc region receptor II Protein, FCGR2B Protein, Fcgr2b Protein, LOC100856270 Protein

Background: CD32B, FCGR2B, FCGRIIB, FCR2B, FCRIIB, IGFR2B, IGFRIIB
Background: Low affinity immunoglobulin gamma Fc receptor IIb, also known as FcγRIIb or CD32b, is a type I integral membrane glycoprotein. CD32b is a member of the immunoglobulin superfamily and is expressed on B cells, subsets of monocytes, macrophages and granulocytes, platelets and mast cells. CD32b binds monomeric IgG with low affinity but is efficient at binding immune complexes and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation. CD32b is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail containing the immunoreceptor tyrosine-based inhibition (ITIM) motif. The product provided only contains the extracellular portion of CD32.

Molecular Weight: 23.8 kDa

UniProt: P31994

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling