Retinoblastoma Binding Protein 4 Protein (RBBP4)

Catalog No. ABIN7520441

Product Details

Target: Retinoblastoma Binding Protein 4 (RBBP4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purpose: Active Recombinant Human RBBP-4 Protein

Sequence: MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPNDD AQFDASHYDS EKGEFGGFGS VSGKIEIEIK INHEGEVNRA RYMPQNPCII ATKTPSSDVL VFDYTKHPSK PDPSGECNPD LRLRGHQKEG YGLSWNPNLS GHLLSASDDH TICLWDISAV PKEGKVVDAK TIFTGHTAVV EDVSWHLLHE SLFGSVADDQ KLMIWDTRSN NTSKPSHSVD AHTAEVNCLS FNPYSEFILA TGSADKTVAL WDLRNLKLKL HSFESHKDEI FQVQWSPHNE TILASSGTDR RLNVWDLSKI GEEQSPEDAE DGPPELLFIH GGHTAKISDF SWNPNEPWVI CSVSEDNIMQ VWQMAENIYN DEDPEGSVDP EGQGS

Specificity: Met1-Ser425

Purity: > 92 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human RBBP4 Protein at 1 μg/mL (100 μL/well) can bind RBBP4 Rabbit pAb with a linear range of 0.976-7.09 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 500 mM NaCl, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: Retinoblastoma Binding Protein 4 (RBBP4)

Alternative Name: RBBP-4 (RBBP4 Products)

Synonyms: NURF55 Protein, RBAP48 Protein, 154659_at Protein, 55 Protein, CAF-1 Protein, CAF1 Protein, CAF1-55 Protein, CAF1p55 Protein, CG4236 Protein, Caf-1 Protein, Caf1/p55 Protein, Caf1p55 Protein, Dmel\\CG4236 Protein, MSI1/RbAp48/CAC3/LIN-53 Protein, NURF Protein, NURF-55 Protein, Nurf Protein, Nurf 55 Protein, Nurf-55 Protein, Nurf55 Protein, P55 Protein, RbAp48 Protein, S(ls)3 Protein, caf-1 Protein, caf1 Protein, caf1 p55 Protein, d-CAF1 Protein, dCAF-1 Protein, dCAF-1 p55 Protein, dNURF Protein, p55 Protein, p55 CAF1 Protein, p55/NURF-55 Protein, p55CAF1 Protein, nurf55 Protein, rbap48 Protein, xrbbp4 Protein, mRbAp48 Protein, RBBP4 Protein, rbb4-2 Protein, zgc:55349 Protein, zgc:77854 Protein, wu:fb33a09 Protein, wu:fb40e10 Protein, RBBP-4 Protein, rbbp4 Protein, M4E13.110 Protein, M4E13_110 Protein, MULTICOPY SUPPRESSOR OF IRA1 3 Protein, NFC3 Protein, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR GROUP C 3 Protein, RB binding protein 4, chromatin remodeling factor Protein, Chromatin assembly factor 1, p55 subunit Protein, retinoblastoma binding protein 4 Protein, retinoblastoma binding protein 4, chromatin remodeling factor Protein, retinoblastoma binding protein 4 L homeolog Protein, Transducin family protein / WD-40 repeat family protein Protein, retinoblastoma binding protein 4 S homeolog Protein, RBBP4 Protein, Caf1-55 Protein, rbbp4 Protein, Rbbp4 Protein, rbbp4.L Protein, MSI3 Protein, rbbp4.S Protein

Background:

Description: Histone-binding protein RBBP4, also known as Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48 and RBBP4, is a nucleus protein which belongs to the WD repeat RBAP46/RBAP48/MSI1 family.It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Name: NURF55, RBAP48, lin-53,RBBP4,RBAP48,lin-53

Gene ID: 5928

UniProt: Q09028

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Stem Cell Maintenance, Chromatin Binding, Protein targeting to Nucleus