Nuclease Protein
Origin: Serratia marcescens
Host: Yeast (Pichia pastoris)
Recombinant
> 95 % by SDS-PAGE.
Active
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- Target See all Nuclease products
- Nuclease
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Serratia marcescens
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Source
- Yeast (Pichia pastoris)
- Purpose
- Active Recombinant Serratia marcescens Nuclease
- Sequence
- DTLESIDNCA VGCPTGGSSN VSIVRHAYTL NNNSTTKFAN WVAYHITKDT PASGKTRNWK TDPALNPADT LAPADYTGAN AALKVDRGHQ APLASLAGVS DWESLNYLSN ITPQKSDLNQ GAWARLEDQE RKLIDRADIS SVYTVTGPLY ERDMGKLPGT QKAHTIPSAY WKVIFINNSP AVNHYAAFLF DQNTPKGADF CQFRVTVDEI EKRTGLIIWA GLPDDVQASL KSKPGVLPEL MGCKN
- Specificity
- Asp22-Asn266
- Purity
- > 95 % by SDS-PAGE.
- Endotoxin Level
- <0.1EU/μg
- Biological Activity Comment
- 1.One unit (U) is defined as the amount of enzyme required to change the absorption value of A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL.|2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37°C for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Solution in 50 % glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
- Storage
- -20 °C
- Storage Comment
- This product is stable at -15°C ~ -25°C for up to 2 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
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- Target
- Nuclease
- Abstract
- Nuclease Products
- Background
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Description: Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.
Name: Endonuclease, Nuclease, nucA, nuc
- Gene ID
- 66715254
- UniProt
- P13717
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