IGFBP4 Protein (His tag)
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- Target See all IGFBP4 Proteins
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IGFBP4 protein is labelled with His tag.
- Purpose
- Active Recombinant Human IGFBP-4 Protein
- Sequence
- MLPLCLVAAL LLAAGPGPSL GDEAIHCPPC SEEKLARCRP PVGCEELVRE PGCGCCATCA LGLGMPCGVY TPRCGSGLRC YPPRGVEKPL HTLMHGQGVC MELAEIEAIQ ESLQPSDKDE GDHPNNSFSP CSAHDRRCLQ KHFAKIRDRS TSGGKMKVNG APREDARPVP QGSCQSELHR ALERLAASQS RTHEDLYIIP IPNCDRNGNF HPKQCHPALD GQRGKCWCVD RKTGVKLPGG LEPKGELDCH QLADSFRE
- Specificity
- Met1-Glu258
- Purity
- > 92 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- 1.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 at 1 μg/mL (100 μL/well) can bind Human IGF1 with a linear range of 1.95-111.32 ng/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP4 Protein at 1 μg/mL (100 μL/well) can bind IGF1 with a linear range of 1.95-122.3ng/mL.
- Top Product
- Discover our top product IGFBP4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
- Alternative Name
- IGFBP-4 (IGFBP4 Products)
- Synonyms
- IGFBP4 Protein, igfbp4 Protein, BP-4 Protein, HT29-IGFBP Protein, IBP4 Protein, IGFBP-4 Protein, AI875747 Protein, Deb2 Protein, IGF-BP4 Protein, IBP-4 Protein, insulin like growth factor binding protein 4 Protein, insulin-like growth factor-binding protein 4 Protein, insulin-like growth factor binding protein 4 Protein, IGFBP4 Protein, LOC100136305 Protein, LOC100194498 Protein, igfbp4 Protein, Igfbp4 Protein
- Background
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Description: IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins . IGFBP-4 binds both insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. It circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. There is mounting evidence that IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.
Name: IGFBP4,BP-4,HT29-IGFBP,IBP4,IGFBP-4
- Gene ID
- 3487
- UniProt
- P22692
- Pathways
- WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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