HTRA2 Protein
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- Target See all HTRA2 Proteins
- HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Active Recombinant Human HTRA2 Protein
- Sequence
- AVPSPPPASP RSQYNFIADV VEKTAPAVVY IEILDRHPFL GREVPISNGS GFVVAADGLI VTNAHVVADR RRVRVRLLSG DTYEAVVTAV DPVADIATLR IQTKEPLPTL PLGRSADVRQ GEFVVAMGSP FALQNTITSG IVSSAQRPAR DLGLPQTNVE YIQTDAAIDF GNSGGPLVNL DGEVIGVNTM KVTAGISFAI PSDRLREFLH RGEKKNSSSG ISGSQRRYIG VMMLTLSPSI LAELQLREPS FPDVQHGVLI HKVILGSPAH RAGLRPGDVI LAIGEQMVQN AEDVYEAVRT QSQLAVQIRR GRETLTLYVT PEVTE
- Specificity
- Ala134-Glu458
- Purity
- > 92 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Biological Activity Comment
- Protease activity demonstrated by HtrA2 cleavage of bovine β-casein . Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.
- Top Product
- Discover our top product HTRA2 Protein
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HtrA Serine Peptidase 2 (HTRA2) (AA 133-358) protein (His tag)
HTRA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
HtrA Serine Peptidase 2 (HTRA2) (full length) protein (His tag)HTRA2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
HtrA Serine Peptidase 2 (HTRA2) (AA 359-458) protein (GST tag)HTRA2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
- Alternative Name
- HTRA2 (HTRA2 Products)
- Synonyms
- CG8464 Protein, DmHtrA2 Protein, Dmel\\CG8464 Protein, HtrA2/Omi Protein, Omi Protein, dOmi Protein, domi Protein, htrA2 Protein, omi Protein, HTRA2 Protein, PRSS25 Protein, OMI Protein, PARK13 Protein, AI481710 Protein, Prss25 Protein, mnd2 Protein, HTRA2-related serine protease Protein, HtrA serine peptidase 2 Protein, HtrA2 Protein, HTRA2 Protein, htra2 Protein, Htra2 Protein
- Background
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Description: The protein is a serine protease. It has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed.
Name: HTRA2,MGCA8,OMI,PARK13,PRSS25, OMI, PARK13, PRSS25
- Gene ID
- 27429
- UniProt
- O43464
- Pathways
- Positive Regulation of Endopeptidase Activity
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