FLT3 Protein (Fc Tag,His tag)

Catalog No. ABIN7535181

Product Details

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FLT3 protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human FLT-3/FLK-2/CD135 Protein

Sequence: NQDLPVIKCV LINHKNNDSS VGKSSSYPMV SESPEDLGCA LRPQSSGTVY EAAAVEVDVS ASITLQVLVD APGNISCLWV FKHSSLNCQP HFDLQNRGVV SMVILKMTET QAGEYLLFIQ SEATNYTILF TVSIRNTLLY TLRRPYFRKM ENQDALVCIS ESVPEPIVEW VLCDSQGESC KEESPAVVKK EEKVLHELFG TDIRCCARNE LGRECTRLFT IDLNQTPQTT LPQLFLKVGE PLWIRCKAVH VNHGFGLTWE LENKALEEGN YFEMSTYSTN RTMIRILFAF VSSVARNDTG YYTCSSSKHP SQSALVTIVE KGFINATNSS EDYEIDQYEE FCFSVRFKAY PQIRCTWTFS RKSFPCEQKG LDNGYSISKF CNHKHQPGEY IFHAENDDAQ FTKMFTLNIR RKPQVLAEAS ASQASCFSDG YPLPSWTWKK CSDKSPNCTE EITEGVWNRK ANRKVFGQWV SSSTLNMSEA IKGFLVKCCA YNSLGTSCET ILLNSPGPFP FIQDN

Specificity: Asn27-Asn541

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human FLT3L at 5 μg/mL (100 μL/well) can bind Human FLT3 with a linear range of 1.95-66.1 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT-3/FLK-2/CD135 (FLT3 Products)

Synonyms: FLT3 Protein, B230315G04 Protein, CD135 Protein, Flk-2 Protein, Flk2 Protein, Flt-3 Protein, Ly72 Protein, wmfl Protein, FLK-2 Protein, FLK2 Protein, STK1 Protein, fms related tyrosine kinase 3 Protein, FMS-like tyrosine kinase 3 Protein, fms-related tyrosine kinase 3 Protein, FLT3 Protein, Flt3 Protein

Background:

Description: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeIII for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.

Name: FLT3,CD135,FLK-2,FLK2,STK1

Gene ID: 2322

UniProt: P36888

Pathways: RTK Signaling