FGFR2 Protein

Catalog No. ABIN7520010

Product Details

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purpose: Active Recombinant Human FGFR-2/KGFR/CD332 Protein

Sequence: PMLAGVSEYE LPEDPKWEFP RDKLTLGKPL GEGCFGQVVM AEAVGIDKDK PKEAVTVAVK MLKDDATEKD LSDLVSEMEM MKMIGKHKNI INLLGACTQD GPLYVIVEYA SKGNLREYLR ARRPPGMEYS YDINRVPEEQ MTFKDLVSCT YQLARGMEYL ASQKCIHRDL AARNVLVTEN NVMKIADFGL ARDINNIDYY KKTTNGRLPV KWMAPEALFD RVYTHQSDVW SFGVLMWEIF TLGGSPYPGI PVEELFKLLK EGHRMDKPAN CTNELYMMMR DCWHAVPSQR PTFKQLVEDL DRILTLTTNE EYLDLSQPLE Q

Specificity: Pro458-Gln778

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 0.21-0.84 ng/mL, corresponding to a specific activity of 1.19x10⁶-4.76x10⁶units/mg.|2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGFR2 at 2 μg/mL (100 μL/well) can bind Recombinant Human FGF1 with a linear range of 0.128-48.3 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, pH 8.0.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Alternative Name: FGFR-2/KGFR/CD332 (FGFR2 Products)

Synonyms: FGFR2 Protein, FGFR2IIIb Protein, bek Protein, bfr-1 Protein, cd332 Protein, cek3 Protein, cfd1 Protein, ect1 Protein, fgfr-2 Protein, jws Protein, k-sam Protein, kgfr Protein, tk14 Protein, tk25 Protein, xfgfr2 Protein, DKFZp469H1521 Protein, BBDS Protein, BEK Protein, BFR-1 Protein, CD332 Protein, CEK3 Protein, CFD1 Protein, ECT1 Protein, JWS Protein, K-SAM Protein, KGFR Protein, TK14 Protein, TK25 Protein, AU043015 Protein, AW556123 Protein, Bek Protein, Fgfr-2 Protein, Fgfr-7 Protein, Fgfr7 Protein, KGFRTr Protein, svs Protein, FGF-R Protein, fgfr72IIIb Protein, FGFR Protein, fc56c05 Protein, wu:fc56c05 Protein, fibroblast growth factor receptor 2 Protein, fibroblast growth factor receptor 2 L homeolog Protein, FGFR2 Protein, LOC100084641 Protein, fgfr2 Protein, Fgfr2 Protein, fgfr2.L Protein

Background:

Description: The protein is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.

Name: BBDS,BEK,BFR-1,CD332,CEK3,CFD1,ECT1,JWS,K-SAM,KGFR,TK14,TK25,FGFR2

Gene ID: 2263

UniProt: P21802

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding