FGF12 Protein (His tag)
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- Target See all FGF12 Proteins
- FGF12 (Fibroblast Growth Factor 12 (FGF12))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This FGF12 protein is labelled with His tag.
- Purpose
- Active Recombinant Human FGF-12 Protein
- Sequence
- MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREPS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T
- Specificity
- Met1-Thr181
- Purity
- > 97 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 1.0 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR4 at 5 μg/mL (100 μL/well) can bind recombinant human FGF12 with a linear range of 35-100 ng/mL.
- Top Product
- Discover our top product FGF12 Protein
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Fibroblast Growth Factor 12 (FGF12) protein
FGF12 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Fibroblast Growth Factor 12 (FGF12) (AA 1-181) protein (GST tag)FGF12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, 1 mM DTT, 300 mM NaCl, pH 7.4.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- FGF12 (Fibroblast Growth Factor 12 (FGF12))
- Alternative Name
- FGF-12 (FGF12 Products)
- Synonyms
- FHF1 Protein, FHF-1 Protein, MGC84150 Protein, FGF12 Protein, fgf12 Protein, zgc:153104 Protein, fhf1 Protein, fgf12b Protein, DKFZp459P139 Protein, FGF12B Protein, AV114868 Protein, FGF-12 Protein, Fhf1 Protein, Fhf1b Protein, fibroblast growth factor 12 Protein, fibroblast growth factor 12 S homeolog Protein, fibroblast growth factor 12a Protein, FGF12 Protein, fgf12.S Protein, fgf12a Protein, fgf12 Protein, Fgf12 Protein
- Background
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Description: The protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted.
Name: FGF12,EIEE47,FGF12B,FHF1
- Gene ID
- 2257
- UniProt
- P61328
- Pathways
- Negative Regulation of Transporter Activity
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