EPH Receptor A2 Protein (EPHA2)

Catalog No. ABIN7533760

Product Details

Target: EPH Receptor A2 (EPHA2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purpose: Active Recombinant Human EphA2/ECK Protein

Sequence: DPNQAVLKFT TEIHPSCVTR QKVIGAGEFG EVYKGMLKTS SGKKEVPVAI KTLKAGYTEK QRVDFLGEAG IMGQFSHHNI IRLEGVISKY KPMMIITEYM ENGALDKFLR EKDGEFSVLQ LVGMLRGIAA GMKYLANMNY VHRDLAARNI LVNSNLVCKV SDFGLSRVLE DDPEATYTTS GGKIPIRWTA PEAISYRKFT SASDVWSFGI VMWEVMTYGE RPYWELSNHE VMKAINDGFR LPTPMDCPSA IYQLMMQCWQ QERARRPKFA DIVSILDKLI RAPDSLKTLA DFDPRVSIRL PSTSGSEGVP FRTVSEWLES IKMQQYTEHF MAAGYTAIEK VVQMTNDDIK RIGVRLPGHQ KRIAYSLLGL KDQVNTVGIP I

Specificity: Asp596-Ile976

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human EphA2 at 1 μg/mL (100 μL/well) can bind Human EFNA1 with a linear range of 4.883 ng/mL-9.206 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EphA2/ECK (EPHA2 Products)

Synonyms: ARCC2 Protein, CTPA Protein, CTPP1 Protein, CTRCT6 Protein, ECK Protein, epha2 Protein, MGC89505 Protein, AW545284 Protein, Eck Protein, Myk2 Protein, Sek-2 Protein, Sek2 Protein, id:ibd5152 Protein, rtk6 Protein, G42 Protein, XE10 Protein, epha2-A Protein, EPH receptor A2 Protein, Eph receptor A2 Protein, epoxide hydrolase Protein, eph receptor A2 a Protein, EPH receptor A2 L homeolog Protein, EPHA2 Protein, Epha2 Protein, epha2 Protein, ephA2 Protein, epha2a Protein, epha2.L Protein

Background:

Description: Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family.The receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration.

Name: ARCC2,CTPA,CTPP1,CTRCT6,ECK,EPHA2

Gene ID: 1969

UniProt: P29317

Pathways: RTK Signaling