CST5 Protein (His tag)
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- Target See all CST5 Proteins
- CST5 (Cystatin D (CST5))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CST5 protein is labelled with His tag.
- Purpose
- Active Recombinant Human Cystatin-D/CST5 Protein
- Sequence
- GSASAQSRTL AGGIHATDLN DKSVQCALDF AISEYNKVIN KDEYYSRPLQ VMAAYQQIVG GVNYYFNVKF GRTTCTKSQP NLDNCPFNDQ PKLKEEEFCS FQINEVPWED KISILNYKCR KV
- Specificity
- Gly21-Val142
- Purity
- > 90 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AM. The IC50 value is <18 nM.
- Top Product
- Discover our top product CST5 Protein
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Cystatin D (CST5) (AA 1-142) protein (GST tag)
CST5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin D (CST5) (AA 26-142) (Active) protein (His tag)CST5 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- CST5 (Cystatin D (CST5))
- Alternative Name
- Cystatin-D/CST5 (CST5 Products)
- Synonyms
- Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein
- Background
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Description: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Cystatins are natural inhibitors of papain-like (family C1) and legumain-related (family C13) cysteine peptidases. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. As a member of type 2 cystatin, cystatin D is a single-domain protein and also has cysteine residues that form disulfide bridges. The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12_x005f
Name: CST5,MGC71922,CST5
- Gene ID
- 1473
- UniProt
- P28325
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