CA2 Protein

Catalog No. ABIN7533772

Product Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Active Recombinant Human Carbonic anhydrase 2 Protein

Sequence: SHHWGYGKHN GPEHWHKDFP IAKGERQSPV DIDTHTAKYD PSLKPLSVSY DQATSLRILN NGHAFNVEFD DSQDKAVLKG GPLDGTYRLI QFHFHWGSLD GQGSEHTVDK KKYAAELHLV HWNTKYGDFG KAVQQPDGLA VLGIFLKVGS AKPGLQKVVD VLDSIKTKGK SADFTNFDPR GLLPESLDYW TYPGSLTTPP LLECVTWIVL KEPISVSSEQ VLKFRKLNFN GEGEPEELMV DNWRPAQPLK NRQIKASFK

Specificity: Ser2-Lys260

Purity: > 90 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its esterase activity. The specific activity is >840 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic anhydrase 2 (CA2 Products)

Synonyms: CG6906 Protein, CT21171 Protein, Dmel\\CG6906 Protein, CA-2 Protein, caII Protein, car2 Protein, ca ii Protein, ca-ii Protein, BETA CA2 Protein, BETA CARBONIC ANHYDRASE 2 Protein, CA18 Protein, CARBONIC ANHYDRASE 18 Protein, CARBONIC ANHYDRASE 2 Protein, T9L3.40 Protein, T9L3_40 Protein, carbonic anhydrase 2 Protein, LOC100135827 Protein, AI131712 Protein, CAII Protein, Ca2 Protein, Car-2 Protein, Ltw-5 Protein, Lvtw-5 Protein, CA-II Protein, CAC Protein, Car2 Protein, CAR3 Protein, Carbonic anhydrase 2 Protein, carbonic anhydrase 2 L homeolog Protein, carbonic anhydrase 2 Protein, carbonic anhydrase 1 Protein, carbonic anhydrase II Protein, carbonic anhydrase, alpha type, periplasmic Protein, CAH2 Protein, ca2.L Protein, ca2 Protein, CA2 Protein, HCAG_04637 Protein, LOC100135827 Protein, LOC100635510 Protein, Car2 Protein, Ca2 Protein, LOC100154873 Protein, LOC101113341 Protein

Background:

Description: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification.

Name: CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282, carbonic anhydrase 2,CA-II,CAC,CAII,Car2,HEL-76,HEL-S-282

Gene ID: 760

UniProt: P00918