Tissue factor Protein (AA 1-252) (His tag)

Catalog No. ABIN7505790

Product Details

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Protein Type: Recombinant

Protein Characteristics: AA 1-252

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This Tissue factor protein is labelled with His tag.

Sequence: Met1-Glu252

Characteristics: A DNA sequence encoding the Rat TF protein (P42533) (Met1-Glu252) was expressed with a C-His.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: Tissue factor (F3 Products)

Synonyms: CD142 Protein, TF Protein, TFA Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, f3 Protein, AA409063 Protein, Cf-3 Protein, Cf3 Protein, tf Protein, zgc:112151 Protein, coagulation factor III, tissue factor Protein, transferrin Protein, coagulation factor IIIa Protein, coagulation factor III Protein, tissue factor Protein, coagulation factor IIIb Protein, coagulation factor III (thromboplastin, tissue factor) S homeolog Protein, F3 Protein, TF Protein, f3a Protein, tf Protein, f3b Protein, f3.S Protein

Background:

Abbreviation: Tissue factor,TF

Target Synonym: Tissue factor,TF,Coagulation factor III,CD antigen CD142,F3,Cf3

Background: Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma. Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis. Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 to 28) and the mature chain (residues 29 to 294). As a type I membrane protein, it contains a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 294).

Molecular Weight:

Calculated MW: 27.61 kDa

Observed MW: 49 kDa

UniProt: P42533

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling