PLA2G7 Protein (AA 1-440) (His tag)

Catalog No. ABIN7505785

Product Details

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Protein Type: Recombinant

Protein Characteristics: AA 1-440

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This PLA2G7 protein is labelled with His tag.

Sequence: Met1-Asn440

Characteristics: A DNA sequence encoding the Rat LpPLA2 protein (Q5M7T7) (Met1-Asn440) was expressed with a C-His.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Alternative Name: Lp-PLA2 (Lp-PLA2 Products)

Synonyms: LDL-PLA2 Protein, LP-PLA2 Protein, PAFAD Protein, PAFAH Protein, Lp-PLA2 Protein, PAF-AH Protein, R75400 Protein, zgc:77563 Protein, phospholipase A2 group VII Protein, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) Protein, PLA2G7 Protein, Pla2g7 Protein, pla2g7 Protein

Background:

Abbreviation: Lp-PLA2,Pla2g7

Target Synonym: Platelet-activating factor acetylhydrolase,Pla2g7,EC:3.1.1.47,rCG_43740

Background: Secretory phopholipase A2 is an enzyme that hydrolyses the Sn-2 ester bond of phospholipids, generating free fatty acids and lysophospholipids. Most secretory PLA2s are stored in cytoplasmic granules and are released in the extracellular environment on appropriate cell activation. Thus, they are present at higher concentration in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic disease, such as acute pancreatitis, rheumatoid arthritis, bronchial asthma, and allergic rhinitis. Also known as Lp-PLA2, PLA2G-VII is a plasma enzyme bound to lipoproteins: 80 % bound to LDL, 15 %-20 % to HDL, and the remainder to VLDL (4-6). It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s, PLA2G?VII has poor specificity toward Sn-2 long chain fatty acids, unless heavily oxidized, and undergoes the catalysis of its substrates in the aqueous phase rather than at the interfacial surface of lipids. Thus, it has high specificity for water-soluble phospholipids in plasma including oxidatively?modified phospholipids and platelet-activating factor (PAF). Because of the latter activity, it is also known as PAF acetylhydrolase (PAF-AH).

Molecular Weight:

Calculated MW: 48.29 kDa

Observed MW: 60 kDa

UniProt: Q5M7T7

Pathways: Peptide Hormone Metabolism