CD8 alpha Protein (AA 1-182) (His tag)

Catalog No. ABIN7505742

Product Details

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Protein Type: Recombinant

Protein Characteristics: AA 1-182

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD8 alpha protein is labelled with His tag.

Sequence: Met1-Gly182

Characteristics: A DNA sequence encoding the Mouse CD8 protein (P01731) (Met1-Gly182) was expressed with a C-His.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a (CD8A Products)

Synonyms: cd8 Protein, LOC100136450 Protein, BB154331 Protein, Ly-2 Protein, Ly-35 Protein, Ly-B Protein, Lyt-2 Protein, zgc:136643 Protein, CD8 Protein, Leu2 Protein, MAL Protein, p32 Protein, RHACD8-4 Protein, CD8a molecule Protein, uncharacterized LOC100125537 Protein, CD8 alpha Protein, CD8 antigen, alpha chain Protein, T-cell surface glycoprotein CD8 alpha chain Protein, Cd8a Protein, LOC100125537 Protein, LOC100136450 Protein, CD8A Protein, cd8a Protein, LOC100126556 Protein

Background:

Abbreviation: CD8a,Lyt2

Target Synonym: BB154331,Ly-2,Ly-35,Ly-B,Lyt-2,T-cell surface glycoprotein CD8 alpha chain,T-cell surface glycoprotein Lyt-2,CD antigen CD8a

Background: T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Molecular Weight:

Calculated MW: 19.91 kDa

Observed MW: 35 kDa

UniProt: P01731

Pathways: TCR Signaling