CD31 Protein (AA 1-601) (His tag)

Catalog No. ABIN7505652

Product Details

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-601

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD31 protein is labelled with His tag.

Sequence: Met 1-Lys 601

Characteristics: A DNA sequence encoding the Human PECAM1/CD31 protein (P16284) (Met 1-Lys 601) was expressed with a C-His.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1 (PECAM1 Products)

Synonyms: CD31 Protein, CD31/EndoCAM Protein, GPIIA' Protein, PECA1 Protein, PECAM-1 Protein, endoCAM Protein, C85791 Protein, Cd31 Protein, Pecam Protein, platelet/endothelial cell adhesion molecule 1 Protein, platelet and endothelial cell adhesion molecule 1 Protein, pecam1 Protein, PECAM1 Protein, Pecam1 Protein

Background:

Abbreviation: PECAM1,CD31

Target Synonym: Platelet endothelial cell adhesion molecule,PECAM-1,EndoCAM,GPIIA,PECA1,CD31,PECAM1

Background: CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a 130 kDa heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 is composed of an extracellular domain (ECD) of 574 amino acids containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. The latter undergoes alternative splicing which generates multiple isoforms showing altered adhesive properties compared to full length CD31. The human CD31 ECD shares 63 % and 61 % aa sequence identity with mouse and rat CD31, respectively. CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP-2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. Besides its role in TEM, CD31 appears to regulate T cell trafficking through a complex coordination of endothelial cell junctions and T cell extravasation.

Molecular Weight:

Calculated MW: 66 kDa

Observed MW: 85 kDa

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization