MICB Protein (AA 1-298) (His tag)

Catalog No. ABIN7505571

Product Details

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-298

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This MICB protein is labelled with His tag.

Application: ELISA

Sequence: Met 1-Gly 298

Characteristics: A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin Level: <1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human MICB-His at 10 ug/ml (100 ul/well) can bind human NKG2D, The EC50 of human NKG2D is 0.52-1. 2 ug/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Alternative Name: MICB (MICB Products)

Synonyms: PERB11.2 Protein, MHC class I polypeptide-related sequence B Protein, MHC class I-related protein-like Protein, MICB Protein, LOC512825 Protein

Background:

Abbreviation: MICB

Target Synonym: PERB11.2

Background: MHC class I polypeptide-related sequence B; also known as MICB; is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA; a closely related protein; both of which contain three extracellular immunoglobulin-like domains; but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen; binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells; CD8+αβ T cells; and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells; but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance; viral infections; and autoimmune diseases.

Molecular Weight:

Calculated MW: 33 kDa

Observed MW: 45-50 kDa

UniProt: Q29980

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response