Retinoblastoma 1 Protein (RB1) (AA 229-450) (His tag)
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- Target See all Retinoblastoma 1 (RB1) Proteins
- Retinoblastoma 1 (RB1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 229-450
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Origin
- Rat
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Retinoblastoma 1 protein is labelled with His tag.
- Sequence
- Arg 229-Met 450
- Characteristics
- A DNA sequence encoding the Rat RB1 protein (P33568) (Arg 229-Met 450) was expressed with N-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product RB1 Protein
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Retinoblastoma 1 (RB1) (AA 371-470) protein (GST tag)
RB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- Retinoblastoma 1 (RB1)
- Alternative Name
- RB1 (RB1 Products)
- Synonyms
- OSRC Protein, RB Protein, p105-Rb Protein, pRb Protein, pp110 Protein, Rb Protein, Rb-1 Protein, P105-RB Protein, PP110 Protein, RB11 Protein, zgc:154147 Protein, RB transcriptional corepressor 1 Protein, RB transcriptional corepressor like 1 Protein, retinoblastoma 1 Protein, RB1 Protein, RBL1 Protein, Rb1 Protein, rb1 Protein
- Background
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Abbreviation: RB1
Target Synonym: OSRC,osteosarcoma,p105-Rb,pp110,pRb,RB,RB1,retinoblastoma 1,retinoblastoma suspectibility protein,retinoblastoma-associated protein
Background: Retinoblastoma 1 protein (RB-1, also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family. Rat RB-1 is 920 amino acids in length. The protein contains a Pocket domain (aa 366-763), which is comprised of two other domains, domain A (aa 366-572) and domain B (aa 632-763), and a ""spacer"" (aa 573-631). The Pocket domain binds to threonine-phosphorylated domain C (aa 763-920), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes. RB-1 is expressed in the retina. The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Defects in RB-1 lead to the childhood cancer retinoblastoma.
- Molecular Weight
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Calculated MW: 24.31 kDa
Observed MW: 32 kDa
- UniProt
- P33568
- Pathways
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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