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PCK1 Protein (AA 1-135) (His tag)

PCK1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN7505500
  • Target See all PCK1 Proteins
    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-135
    Origin
    • 5
    • 3
    • 2
    Rat
    Source
    • 5
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This PCK1 protein is labelled with His tag.
    Sequence
    Met 1-Lys 135
    Characteristics
    A DNA sequence encoding the Rat PEPCK-C protein (P07379) (Met 1-Lys 135) was expressed with a N-His tag.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Top Product
    Discover our top product PCK1 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Lyophilized from sterile PBS, pH 7.4.
    Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Expiry Date
    12 months
  • Target
    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
    Alternative Name
    PEPCK-C (PCK1 Products)
    Synonyms
    PEPCK-C Protein, PEPCK1 Protein, PEPCKC Protein, PEPCK Protein, PEPCK-M Protein, PEPCK2 Protein, 1810010O14Rik Protein, 9130022B02Rik Protein, cb856 Protein, cb924 Protein, fj93h11 Protein, zgc:63869 Protein, wu:fc51c05 Protein, wu:fj93h11 Protein, pepck-c Protein, pepck1 Protein, pepckc Protein, PCK1 Protein, PPCK1 Protein, AI265463 Protein, Pck-1 Protein, GTP Protein, PCK Protein, Pepck Protein, RATPEPCK Protein, Ppc1C Protein, PHOSPHOENOLPYRUVATE CARBOXYKINASE Protein, T28I19.150 Protein, T28I19_150 Protein, phosphoenolpyruvate carboxykinase 1 Protein, 143299_at Protein, CG10924 Protein, CG17725 Protein, Dmel\\CG17725 Protein, Dromel_CG17725_FBtr0086701_pepck_mORF Protein, dPEPCK Protein, pepck Protein, PEPC Protein, PEPCase Protein, ppc Protein, phosphoenolpyruvate carboxykinase 1 Protein, phosphoenolpyruvate carboxykinase 2, mitochondrial Protein, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Protein, phosphoenolpyruvate carboxykinase 1 (soluble) Protein, phosphoenolpyruvate carboxykinase 1 S homeolog Protein, phosphoenolpyruvate carboxykinase, cytosolic [GTP] Protein, phosphoenolpyruvate carboxykinase 1, cytosolic Protein, phosphoenolpyruvate carboxylase 7 Protein, Phosphoenolpyruvate carboxykinase Protein, phosphoenolpyruvate carboxylase Protein, PCK1 Protein, PCK2 Protein, Pck2 Protein, pck1 Protein, pck1.S Protein, LOC100634531 Protein, Pck1 Protein, pep7 Protein, Pepck Protein, LOC107777405 Protein
    Background

    Abbreviation: PEPCK-C

    Target Synonym: cytosolic [GTP],PCK1,PEP carboxykinase,PEPCK-C,PEPCKC,Phosphopyruvate carboxylase

    Background: PCK1 (Phosphoenolpyruvate carboxykinase 1, also PEPCK-C [cytosolic]) is a monomeric, 67-68 kDa member of the PEP carboxykinase family of enzymes. It is expressed in postnatal cells such as mammary epithelium, white and brown adipocytes, skeletal muscle cells and hepatocytes. PCK1 has multiple functions, some of which are cell-specific. In particular, PCK1 has both cataplerotic (Greek: to fill down, or remove) and anaplerotic (to fill up, or replace) activity, where it removes and replaces elements of the TCA cycle. It is also gluconeogenic, and promotes glucose formation via PEP generation. Finally, it is glyceroneogenic, creating glycerol-3-phosphate that is used to reesterify and store just-released free fatty acids in adipocytes. It contains one kinase domain (aa 27-615), and two potential acetylation sites at Lys70 and 71. There are four potential splice forms. Two have alternative start sites at Met460 and Met315, while two others show a deletion of aa 34-546, plus a three aa substitution for aa 85-204, respectively.

    Molecular Weight

    Calculated MW: 14.74 kDa

    Observed MW: 15 kDa

    UniProt
    P07379
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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