TPO (AA 22-195) protein (His tag)
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- Target
- TPO
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-195
- Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Sequence
- Ser 22-Phe 195
- Characteristics
- A DNA sequence encoding the Mouse TPO/Thpo protein (P40226) (Ser 22-Phe 195) was expressed with a N-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
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TPO protein (His tag)
Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- TPO
- Background
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Abbreviation: TPO,Thpo
Target Synonym: Thrombopoietin,C-mpl ligand,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,Myeloproliferative leukemia virus oncogene ligand,THPO
Background: Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets.Mature mouse Tpo shares 71 % and 81 % aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
- Molecular Weight
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Calculated MW: 19.03 kDa
Observed MW: 22 kDa
- UniProt
- P40226
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