Caspase 8 Protein (CASP8) (AA 219-376) (His tag)
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- Target See all Caspase 8 (CASP8) Proteins
- Caspase 8 (CASP8)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 219-376
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Caspase 8 protein is labelled with His tag.
- Sequence
- Ser 219-Gly 376
- Characteristics
- A DNA sequence encoding the Mouse CASP8 protein (O89110) (Ser219-Gly376) was expressed with a N-His .
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product CASP8 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- Caspase 8 (CASP8)
- Alternative Name
- Caspase-8 (CASP8 Products)
- Synonyms
- casp8-A Protein, xCaspase-8 Protein, casp8 Protein, CASP8 Protein, CASP-8 Protein, FLICE Protein, MACH Protein, Mch5 Protein, ALPS2B Protein, CAP4 Protein, Casp-8 Protein, MCH5 Protein, caspase-8 Protein, zgc:92075 Protein, caspase 8 L homeolog Protein, death related ced-3/Nedd2-like protein Protein, caspase 8 Protein, caspase-8 Protein, caspase-8-like Protein, caspase 8, apoptosis-related cysteine peptidase Protein, casp8.L Protein, LOC661095 Protein, CASP8 Protein, LOC5563818 Protein, casp8 Protein, LOC100222284 Protein, Casp8 Protein
- Background
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Abbreviation: Caspase-8,CASP8
Target Synonym: CASP8_MOUSE,Caspase-8,Casp8,EC:3.4.22.61
Background: Caspase-8 (Cysteine-aspartic acid protease 8/Casp8a, also named MCH5, FLICA and MACH alpha 1) is a 28 kDa member of the peptidase C14A family of enzymes. It is widely expressed and is considered an initiating caspase for the apoptotic cascade. Caspase-8 acts on a wide variety of substrates, including procaspases?3, 4, 6, 7, 9 and 10, c?FLIPL and procaspase-8 itself. Human procaspase?8a is a 54?56 kDa, 479 amino acid (aa) protein. It contains two N?terminal death domains (aa 1?177), followed by a catalytic site that utilizes His317Gly318 plus Cys360. Normally, it is an inactive, cytosolic monomer. But following death?domain (DD) containing receptor oligomerization, Caspase?8 is recruited to the death-inducing signaling complex (DISC) that forms around the death domains of the oligomerized receptor.
- Molecular Weight
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Calculated MW: 17.27 kDa
Observed MW: 20 kDa
- UniProt
- O89110
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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