CNN1 Protein (AA 1-297) (His tag)

Catalog No. ABIN7505318

Product Details

Target: CNN1 (Calponin 1 (CNN1))

Protein Type: Recombinant

Protein Characteristics: AA 1-297

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CNN1 protein is labelled with His tag.

Sequence: Met 1-Ala 297

Characteristics: A DNA sequence encoding the Human CNN1 protein (P51911) (Met 1-Ala 297) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CNN1 (Calponin 1 (CNN1))

Alternative Name: CNN1 (CNN1 Products)

Synonyms: CN Protein, CnnI Protein, SMCC Protein, Sm-Calp Protein, XclpH3 Protein, clpH3 Protein, cnn2 Protein, calponin 1 Protein, calponin 1, basic, smooth muscle L homeolog Protein, cnn1 Protein, Cnn1 Protein, CNN1 Protein, cnn1.L Protein

Background:

Abbreviation: CNN1

Target Synonym: Calponin-1,CNN1,Calponin H1,smooth muscle

Background: CNN-1 (Calponin 1 [calcium and calmodulin-binding troponin T-like protein], also Calponin basic, CaP and Calponin H1) is a 32-36 kDa cytoplasmic member of the calponin family of proteins. Although reportedly expressed in fibroblasts and endothelial cells, it actually appears to be restricted to smooth muscle and smooth muscle-like cells such as myoepithelium and myofibroblasts in the adult. CNN-1 interacts with F-actin in a phosphorylation-dependent manner. When nonphosphorylated, CNN-1 blocks actomyosin ATPase activity, contributing to the stabilization of actin stress fiber bundles. Thus, CNN-1 expression inhibits cell motility and the formation of podosomes. Human CNN-1 is 297 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 30-127), and three consecutive calponin-like repeats (aa 164-268). The repeats are suggested to mediate actin binding. There are five potential Ser/Thr phosphorylation sites. Full-length human CNN-1 shares 97 % aa sequence identity with mouse CNN-1.

Molecular Weight:

Calculated MW: 32.56 kDa

Observed MW: 35 kDa

UniProt: P51911