Cytokeratin 19 Protein (AA 80-400) (His tag)
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- Target See all Cytokeratin 19 (KRT19) Proteins
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 80-400
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Cytokeratin 19 protein is labelled with His tag.
- Sequence
- Glu 80-Leu 400
- Characteristics
- A DNA sequence encoding the Human Cytokein19 protein (P08727) (Glu 80-Leu 400) was expressed with a N-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product KRT19 Protein
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Keratin 19 (KRT19) (AA 310-399) protein (His tag)
KRT19 Origin: Dog Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Keratin 19 (KRT19) (AA 1-400) protein (GST tag)KRT19 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Keratin 19 (KRT19) protein (DYKDDDDK Tag)KRT19 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Keratin 19 (KRT19) protein (Myc-DYKDDDDK Tag)KRT19 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
- Abstract
- KRT19 Products
- Synonyms
- CK19 Protein, K19 Protein, K1CS Protein, AI663979 Protein, EndoC Protein, Krt-1.19 Protein, Krt1-19 Protein, Ka19 Protein, k19 Protein, ck19 Protein, k1cs Protein, krt9 Protein, krt15 Protein, MGC76282 Protein, GK-19 Protein, MGC83069 Protein, KRT19 Protein, keratin 19 Protein, keratin 19 L homeolog Protein, keratin, type I cytoskeletal 19 Protein, KRT19 Protein, Krt19 Protein, krt19 Protein, krt19.L Protein, LOC100344434 Protein, LOC101117946 Protein
- Background
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Abbreviation: Cytokein19
Target Synonym: Keratin,type I cytoskeletal 19,KRT19,Keratin-19 (K19)
Background: Cytokeratin 19 (Keratin, type I cytoskeletal 19, also KRT-19, CK19 and Keratin-19) is a 40-45 kDa, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 (plus KRT-5 and -7) to form 8-10 nm filaments in epidermal stem cells, secretory gland (sweat, mammary, bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79) and a subsequent "rod" region (aa 80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses at least 5 utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa 2-400) shares 82 % aa sequence identity with mouse Cytokeratin 19.
- Molecular Weight
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Calculated MW: 35.2 kDa
Observed MW: 38 kDa
- UniProt
- P08727
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