E2F2 Protein (AA 65-437) (His tag)

Catalog No. ABIN7505314

Product Details

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Protein Type: Recombinant

Protein Characteristics: AA 65-437

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This E2F2 protein is labelled with His tag.

Sequence: Ala 65-Asn 437

Characteristics: A DNA sequence encoding the Human E2F2 protein (Q14209) (Ala 65-Asn 437) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F2 (E2F2 Products)

Synonyms: E2F-2 Protein, CG1071 Protein, Dmel\\CG1071 Protein, E2F Protein, E2F2 Protein, dE2F-2 Protein, dE2F2 Protein, dE2f2 Protein, de2f2 Protein, e2f2 Protein, 9230110J10 Protein, E130207A07 Protein, fb40b03 Protein, wu:fb40b03 Protein, E2F transcription factor 2 Protein, si:ch211-160f23.5 Protein, E2F2 Protein, E2f2 Protein, si:ch211-160f23.5 Protein

Background:

Abbreviation: E2F2

Target Synonym: Transcription factor E2F2,E2F2,

Background: E2F-2 (viral E2-associated factor 2) is a 50?55 kDa member of the E2F/DP family of transcription factors. It is principally expressed by placenta, and forms a DNA?activating E2F heterodimeric complex with DP-1 or -2. This complex, when active, promotes cell cycle progression. In quiescent cells, association with the retinoblastoma-tumor suppressor gene product termed pRB suppresses its activity. Human E2F?2 is 437 amino acids (aa) in length and contains a CDK2 binding region (aa 65?105), a DNA?binding domain (aa 107?196), a dimerization segment (aa 197?289), a transactivation region (aa 359?437), and a pRB binding domain (aa 410?427). There are two potential alternate start sites at Met197 and Met342, and one splice variant that shows a two aa substitution for aa 349?437.

Molecular Weight:

Calculated MW: 40.92 kDa

Observed MW: 55 kDa

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication