Leptin Receptor Protein (LEPR) (AA 22-333) (His tag)
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- Target See all Leptin Receptor (LEPR) Proteins
- Leptin Receptor (LEPR)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-333
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Leptin Receptor protein is labelled with His tag.
- Sequence
- Phe 22-Val 333
- Characteristics
- A DNA sequence encoding the Human Ob-R protein (P48357) (Phe 22-Val 333) was expressed with a N-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product LEPR Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- Leptin Receptor (LEPR)
- Alternative Name
- LEPR (LEPR Products)
- Synonyms
- CD295 Protein, LEP-R Protein, LEPRD Protein, OB-R Protein, OBR Protein, obr Protein, cd295 Protein, ob-rb Protein, Fa Protein, LEPROT Protein, Leprb Protein, Modb1 Protein, OB-RGRP Protein, Obr Protein, db Protein, diabetes Protein, obese-like Protein, obl Protein, ObRb Protein, Ob-R Protein, Ob-RM Protein, lepr Protein, leptin receptor Protein, leptin receptor S homeolog Protein, LEPR Protein, lepr Protein, Lepr Protein, lepr.S Protein
- Background
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Abbreviation: LEPR,CD295
Target Synonym: Leptin receptor,LEP-R,HuB219,OB receptor,OB-R,CD295,LEPR,DB,OBR
Background: Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is a transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms, Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.
- Molecular Weight
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Calculated MW: 34.21 kDa
Observed MW: 35 kDa
- UniProt
- P48357
- Pathways
- JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour
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