Phenylalanine Hydroxylase Protein (AA 2-452) (His tag)

Catalog No. ABIN7505271

Product Details

Target: Phenylalanine Hydroxylase

Protein Type: Recombinant

Protein Characteristics: AA 2-452

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Phenylalanine Hydroxylase protein is labelled with His tag.

Sequence: Ser 2-Lys 452

Characteristics: A DNA sequence encoding the Human PAH protein (P00439) (Ser 2-Lys 452) was expressed with a C-His&N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Phenylalanine Hydroxylase

Alternative Name: PAH (Phenylalanine Hydroxylase Products)

Synonyms: cb877 Protein, wu:fb02f10 Protein, zgc:65981 Protein, GB18494 Protein, PAH Protein, pku Protein, pku1 Protein, DDBDRAFT_0206188 Protein, DDBDRAFT_0231664 Protein, DDB_0206188 Protein, DDB_0231664 Protein, AW106920 Protein, PH Protein, PKU Protein, PKU1 Protein, phenylalanine-4-hydroxylase Protein, phenylalanine hydroxylase Protein, protein henna Protein, phenylalanine 4-monooxygenase Protein, pah Protein, LOC408622 Protein, PAH Protein, CpipJ_CPIJ002149 Protein, Pah Protein

Background:

Abbreviation: PAH

Target Synonym: PH,PKU,PKU1

Background: PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family. It contains 1 ACT domain, N-terminal region of PAH is thought to contain allosteric binding sites for phenylalanine and to constitute an "inhibitory" domain that regulates the activity of a catalytic domain in the C-terminal portion of the molecule. In humans, PAH is expressed both in the liver and the kidney, and there is some indication that it may be differentially regulated in these tissues. PAH catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the pterin-dependent amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin and a non-heme iron for catalysis. Defects in PAH are the cause of phenylketonuria (PKU). PKU is an autosomal recessive inborn error of phenylalanine metabolism, due to severe phenylalanine hydroxylase deficiency. It is characterized by blood concentrations of phenylalanine persistently above 1200 mumol.

Molecular Weight:

Calculated MW: 49.5 kDa

Observed MW: 50 kDa

UniProt: P00439