Pyrophosphatase (Inorganic) 1 (PPA1) (AA 2-289) protein (His tag)

Catalog No. ABIN7505261

Product Details

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Protein Type: Recombinant

Protein Characteristics: AA 2-289

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Sequence: Ser 2-Asn 289

Characteristics: A DNA sequence encoding the Human PPA1 protein (Q15181) (Ser 2-Asn 289) was expressed with a N-His&C-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Alternative Name: PPA1 (PPA1 Products)

Synonyms: ppa Protein, SCE9.16 Protein, PSPTO0722 Protein, AO090005001437 Protein, IOPPP Protein, PP Protein, PP1 Protein, SID6-8061 Protein, 2010317E03Rik Protein, Pyp Protein, Pp Protein, inorganic pyrophosphatase Protein, Inorganic pyrophosphatase Protein, pyrophosphatase (inorganic) 1 L homeolog Protein, pyrophosphatase (inorganic) 1 Protein, ppa Protein, SCO3409 Protein, ppa-1 Protein, APE_1692.1 Protein, AOR_1_2492174 Protein, Celal_2154 Protein, Celly_1958 Protein, Dester_1338 Protein, Marky_0054 Protein, Halhy_5834 Protein, ppa1.L Protein, PPA1 Protein, Ppa1 Protein

Target Type: Viral Protein

Background:

Abbreviation: PPA1

Target Synonym: Inorganic pyrophosphatase,PPA1,Pyrophosphate phospho-hydrolase (PPase)

Background: PPA1 (Inorganic pyrophosphatase 1, also PPase and IOPPP) is a 32-36 kDa cytoplasmic member of the PPase family of enzymes. It is ubiquitously expressed, and acts on di- (or pyro) phosphate, generating orthophosphate in a Mg+-dependent manner. This activity can both generate energy for cells, or in the case of osteoblasts, provide raw material for calcification. The consumption of pyrophosphate may also remove inhibitors of enzymes such as guanylyl cyclase, and PPA1 itself is also reported to stimulate gene expression. Human PPA1 is 289 amino acids (aa) in length. There is one pyrophosphatase domain between aa 42-255, and two utilized acetylation sites at Lys 57 and Lys228. PPA1 is known to form homodimers. There is one potential alternative start site at Met46. Full-length human PPA1 shares 94 % aa sequence identity with mouse PPA1.

Molecular Weight:

Calculated MW: 31.57 kDa

Observed MW: 36 kDa

UniProt: Q15181