RAC2 Protein (AA 1-189) (His tag)

Catalog No. ABIN7505249

Product Details

Target: RAC2 (Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2))

Protein Type: Recombinant

Protein Characteristics: AA 1-189

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RAC2 protein is labelled with His tag.

Sequence: Met 1-Cys 189

Characteristics: A DNA sequence encoding the Human Rac2 protein (P15153) (Met 1-Cys 189) was expressed with a N-His&C-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RAC2 (Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2))

Alternative Name: Rac2 (RAC2 Products)

Synonyms: rac2 Protein, AI323801 Protein, AI452260 Protein, EN-7 Protein, Gx Protein, HSPC022 Protein, p21-Rac2 Protein, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) Protein, RAS-related C3 botulinum substrate 2 Protein, Rac family small GTPase 2 Protein, rac2 Protein, Rac2 Protein, RAC2 Protein

Background:

Abbreviation: Rac2

Target Synonym: EN-7;Gx;HSPC022;p21-Rac2

Background: Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.

Molecular Weight:

Calculated MW: 20.68 kDa

Observed MW: 25 kDa

UniProt: P15153

Pathways: RTK Signaling, VEGF Signaling