RAD1 Protein (AA 1-282) (His tag)
-
- Target See all RAD1 Proteins
- RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-282
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RAD1 protein is labelled with His tag.
- Sequence
- Met 1-Ser 282
- Characteristics
- A DNA sequence encoding the Human RAD1 protein (O60671) (Met 1-Ser 282) was expressed with a N-His&C-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product RAD1 Protein
-
Cell Cycle Checkpoint Protein RAD1 (RAD1) (AA 1-282) protein (GST tag)
RAD1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Cell Cycle Checkpoint Protein RAD1 (RAD1) (AA 1-90) protein (GST tag)RAD1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Cell Cycle Checkpoint Protein RAD1 (RAD1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)RAD1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
-
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
-
- Target
- RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))
- Alternative Name
- RAD1 (RAD1 Products)
- Synonyms
- HRAD1 Protein, REC1 Protein, zgc:56502 Protein, zgc:77779 Protein, RAD1 Protein, xrad1 Protein, DKFZp459H1127 Protein, RAD1 checkpoint DNA exonuclease Protein, RAD1 homolog (S. pombe) Protein, RAD1 Protein, Rad1 Protein, rad1 Protein
- Background
-
Abbreviation: RAD1
Target Synonym: Cell cycle checkpoint protein RAD1,RAD1, DNA repair exonuclease rad1 homolog,Rad1-like DNA damage checkpoint protein
Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
- Molecular Weight
-
Calculated MW: 30.91 kDa
Observed MW: 35 kDa
- UniProt
- O60671
- Pathways
- M Phase
-
