RUNX3 Protein (AA 1-415) (His tag)

Catalog No. ABIN7505242

Product Details

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Protein Type: Recombinant

Protein Characteristics: AA 1-415

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RUNX3 protein is labelled with His tag.

Sequence: Met 1-Tyr 415

Characteristics: A DNA sequence encoding the Human RUNX3 protein (Q13761) (Met 1-Tyr 415) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Alternative Name: RUNX3 (RUNX3 Products)

Synonyms: runxb Protein, RUNX3 Protein, CBFA3 Protein, frrunx3 Protein, runx3 Protein, aml2 Protein, cbfa3 Protein, pebp2ac Protein, AML2 Protein, Cbfa3 Protein, Pebp2a3 Protein, PEBP2aC Protein, runt-related transcription factor 3 Protein, runt related transcription factor 3 Protein, runt related transcription factor 3 L homeolog Protein, runx3 Protein, RUNX3 Protein, runx3.L Protein, Runx3 Protein

Background:

Abbreviation: RUNX3

Target Synonym: Runt-related transcription factor 3,RUNX3,Acute myeloid leukemia 2 protein,Core-binding factor subunit alpha-3,Oncogene AML-2,

Background: RUNX3, also called CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The runt domain (aa 54-186) is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91 % aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.

Molecular Weight:

Calculated MW: 45.54 kDa

Observed MW: 50 kDa

UniProt: Q13761