Ataxin 3 Protein (ATXN3) (AA 1-370) (His tag)

Catalog No. ABIN7505219

Product Details

Target: Ataxin 3 (ATXN3)

Protein Type: Recombinant

Protein Characteristics: AA 1-370

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Ataxin 3 protein is labelled with His tag.

Sequence: Met 1-Lys 370

Characteristics: A DNA sequence encoding the Human ATXN3 protein (P54252-2) (Met 1-Lys 370) was expressed with a C-His and N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Ataxin 3 (ATXN3)

Alternative Name: Ataxin-3 (ATXN3 Products)

Synonyms: Mjd Protein, zgc:56323 Protein, ATXN3 Protein, at3 Protein, jos Protein, mjd Protein, atx3 Protein, mjd1 Protein, sca3 Protein, ataxin-3 Protein, MJD1 Protein, MGC83584 Protein, AT3 Protein, ATX3 Protein, JOS Protein, MJD Protein, SCA3 Protein, 2210008M02Rik Protein, AI463012 Protein, AI647473 Protein, Sca3 Protein, Rsca3 Protein, ataxin 3 Protein, ataxin 3 L homeolog Protein, ataxin-3 Protein, atxn3 Protein, ATXN3 Protein, atxn3.L Protein, MICPUN_104046 Protein, MICPUCDRAFT_46658 Protein, Atxn3 Protein

Background:

Abbreviation: Ataxin-3,ATXN3

Target Synonym: Ataxin-3,ATXN3,Machado-Joseph disease protein1,Spinocerebellar ataxia type 3 protein

Background: Ataxin-3 is a ubiquitously expressed nuclear protein that serves as a deubiquitinating enzyme (DUB) involved in protein homeostasis and quality control, myogenesis and degradation of misfolded chaperone substrates. As a DUB, it has two distinct features. Its N-terminal Josephin domain confers cysteine protease activity to Ataxin-3 which is important for hydrolyzing ubiquitin (Ub) linkages. The second important feature is its three Ub-interacting motifs (UIMs) through which it binds Ub conjugates and ubiquitinated proteins and bring them into proximity to trim or edit specific linkages within these Ub conjugates. It binds long poly-Ub chains and trims them. However, it has weak or no activity against chains of 4 or less Ub.

Molecular Weight:

Calculated MW: 40.59 kDa

Observed MW: 50 kDa

UniProt: P54252-2