GADD45A Protein (AA 1-165) (His tag)

Catalog No. ABIN7505208

Product Details

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Protein Type: Recombinant

Protein Characteristics: AA 1-165

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GADD45A protein is labelled with His tag.

Sequence: Met 1-Arg 165

Characteristics: A DNA sequence encoding the Human GADD45A protein (P24522 ) (1M-165R) was expressed with N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Alternative Name: GADD45A (GADD45A Products)

Synonyms: MGC53491 Protein, zgc:91795 Protein, gadd45a Protein, GADD45A Protein, ddit1 Protein, gadd45 Protein, GADD45 Protein, AA545191 Protein, Ddit1 Protein, DDIT1 Protein, Gadd45 Protein, growth arrest and DNA damage inducible alpha L homeolog Protein, growth arrest and DNA-damage-inducible, alpha, b Protein, growth arrest and DNA damage inducible alpha Protein, growth arrest and DNA damage-inducible protein 45 Protein, growth arrest and DNA-damage-inducible 45 alpha Protein, growth arrest and DNA-damage-inducible, alpha Protein, gadd45a.L Protein, gadd45ab Protein, GADD45A Protein, gadd45a Protein, GADD45 Protein, Gadd45a Protein

Background:

Abbreviation: GADD45A

Target Synonym: Growth arrest and DNA damage-inducible protein GADD45 alpha,GADD45A,DNA damage-inducible transcript 1 protein,

Background: GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33?61 and 133?165. There is one splice variant that shows a Ser substitution for aa 14?49. GADD45 alpha is 55 % aa identical to GADD45-beta and -gamma, and shares 94 % aa identity with mouse GADD45 alpha.

Molecular Weight:

Calculated MW: 18.04 kDa

Observed MW: 23 kDa

UniProt: P24522

Pathways: p53 Signaling, Cell Division Cycle