TGFB3 Protein (AA 301-412) (His tag)

Catalog No. ABIN7505177

Product Details

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Protein Type: Recombinant

Protein Characteristics: AA 301-412

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TGFB3 protein is labelled with His tag.

Sequence: Ala 301-Ser 412

Characteristics: A DNA sequence encoding the Human TGF-beta 3/TGFB3 protein (P10600) (Ala 301-Ser 412) was expressed with N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Alternative Name: TGF-beta 3 (TGFB3 Products)

Synonyms: TGFB3 Protein, ARVD Protein, TGF-beta3 Protein, Tgfb-3 Protein, TGF-B3 Protein, TGFbeta3 Protein, wu:fc78g09 Protein, zgc:92058 Protein, transforming growth factor beta 3 Protein, transforming growth factor, beta 3 Protein, TGFB3 Protein, Tgfb3 Protein, tgfb3 Protein

Background:

Abbreviation: TGF-beta 3,TGFB3

Target Synonym: ARVD,ARVD1,LDS5,RNHF,TGFbeta 3,transforming growth factor beta-3

Background: TGF?-beta 3 (transforming growth factor-beta 3) is a member of a TGF?-beta superfamily subgroup that is defined by their structural and functional similarities. TGF-beta 3 and its closely related proteins, TGF-beta 1 and ? beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-?mesenchymal transition. The non-redundant biological effects of TGF-? beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development. Human TGF?-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-?like convertase to generate a 220 aa latency-?associated peptide (LAP) and a 112 aa mature TGF?-beta 3. Mature human TGF-? beta 3 shows 100 % , 99 %, and 98 % aa identity with mouse/dog/horse, rat, and pig TGF-? beta 3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases. It can also be activated by extreme pH and reactive oxygen species. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-? beta RI or ALK-?1, to modulate transcription through Smad phosphorylation. The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-? beta RIII and endoglin) and the subsequent initiation of Smad--dependent or -independent signaling pathways.

Molecular Weight:

Calculated MW: 12.21 kDa

Observed MW: 30 kDa

UniProt: P10600

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus