IL-18 Protein (AA 116-193) (His-Avi Tag)
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- Target See all IL-18 (IL18) Proteins
- IL-18 (IL18) (Interleukin 18 (IL18))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 116-193
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL-18 protein is labelled with His-Avi Tag.
- Sequence
- Ile 116-Asp 193
- Characteristics
- A DNA sequence encoding the Human IL18 protein (Q14116) (Ile 116-Asp 193) was expressed with a C-Avi-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product IL18 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- IL-18 (IL18) (Interleukin 18 (IL18))
- Alternative Name
- IL18 (IL18 Products)
- Synonyms
- Igif Protein, Il-18 Protein, IL-18 Protein, IGIF Protein, ChIL-18 Protein, IL-1g Protein, IL1F4 Protein, IL18 Protein, interleukin 18 Protein, Il18 Protein, IL18 Protein
- Background
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Abbreviation: IL18 C terminal
Target Synonym: IGIF;IL-18;IL-1g;IL1F4;Interleukin 18
Background: Interleukin-18 (IL-18; also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production; and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine; and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active; was found to increase IgE production in a CD4+ T cells-; IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus; caspase-1 and IL-18 may be critical in regulation of IgE production in vivo; providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was; in turn; upregulated by TNF-α and IL-1β; suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides; synergistic combinations of IL-18; IL-12; and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
- Molecular Weight
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Calculated MW: 8.47 kDa
Observed MW: 12 kDa
- UniProt
- Q14116
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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