BACE1 Protein (AA 170-428) (His tag)

Catalog No. ABIN7505103

Product Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Protein Type: Recombinant

Protein Characteristics: AA 170-428

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BACE1 protein is labelled with His tag.

Sequence: Phe 170-Thr 428

Characteristics: A DNA sequence encoding the Human BACE1 protein (P56817) (Phe 170-Thr 428) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1 (BACE1 Products)

Synonyms: ASP2 Protein, BACE Protein, HSPC104 Protein, C76936 Protein, Bace Protein, MGC145931 Protein, BACE1 Protein, zgc:77409 Protein, beta-secretase 1 Protein, beta-site APP cleaving enzyme 1 Protein, beta-site APP-cleaving enzyme 1 Protein, BACE1 Protein, Bace1 Protein, bace1 Protein

Background:

Abbreviation: BACE1

Target Synonym: ASP2,BACE,HSPC104,BACE1,BACE,KIAA1149,Beta-secretase 1

Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

Molecular Weight:

Calculated MW: 28.38 kDa

Observed MW: 31 kDa

UniProt: P56817