GRP78 Protein (AA 261-470) (His tag)
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- Target See all GRP78 (HSPA5) Proteins
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 261-470
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GRP78 protein is labelled with His tag.
- Sequence
- Gln 261-Glu 470
- Characteristics
- A DNA sequence encoding the Human GRP78 protein (P11021) (Gln 261-Glu 470) was expressed with a N-His tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product HSPA5 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
- Alternative Name
- GRP78 (HSPA5 Products)
- Synonyms
- GRP78 Protein, BiP Protein, GRP-78 Protein, grp78 Protein, hspa5a Protein, BIP Protein, MIF2 Protein, AL022860 Protein, AU019543 Protein, Bip Protein, D2Wsu141e Protein, D2Wsu17e Protein, Grp78 Protein, Hsce70 Protein, SEZ-7 Protein, Sez7 Protein, baffled Protein, mBiP Protein, cb865 Protein, fb60h09 Protein, fi36d04 Protein, wu:fb60h09 Protein, wu:fi36d04 Protein, zgc:55994 Protein, zgc:77606 Protein, 78 kDa glucose-regulated protein Protein, heat shock protein family A (Hsp70) member 5 Protein, BiP/GRP78 Protein, glucose-regulated protein 78 Protein, putative glucose-regulated protein 78 Protein, Hsp70 family ATPase KAR2 Protein, heat shock protein family A (Hsp70) member 5 S homeolog Protein, heat shock 70 kDa protein 5a Protein, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Protein, heat shock protein 5 Protein, heat shock protein family A member 5 Protein, CpipJ_CPIJ003550 Protein, HSPA5 Protein, grp78 Protein, LOC100533358 Protein, BiP/grp78 Protein, Tc00.1047053506585.40 Protein, Tb11.02.5450 Protein, Tb11.02.5500 Protein, LMJF_28_1200 Protein, KAR2 Protein, LOC100135840 Protein, hspa5.S Protein, hspa5 Protein, Hspa5 Protein
- Background
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Abbreviation: GRP78
Target Synonym: HSPA5,BIP,GRP78,HEL-S-89n,MIF2
Background: GRP78 (Glucose-regulated protein 78 kDa, also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding, extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145?245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 1?10), and multiple single aa substitituion.
- Molecular Weight
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Calculated MW: 22.99 kDa
Observed MW: 30 kDa
- UniProt
- P11021
- Pathways
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
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