DNASE1 Protein (AA 23-259) (His tag)

Catalog No. ABIN7505085

Product Details

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Protein Type: Recombinant

Protein Characteristics: AA 23-259

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DNASE1 protein is labelled with His tag.

Sequence: Leu 23-Ala 259

Characteristics: A DNA sequence encoding the Human DNase-I protein (P24855) (Leu 23-Ala 259) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Alternative Name: DNase-I (DNASE1 Products)

Synonyms: LOC397802 Protein, DNL1 Protein, DRNI Protein, AI788650 Protein, DNaseI Protein, Dnl1 Protein, dnase1-A Protein, zgc:92440 Protein, DNase I Protein, deoxyribonuclease 1 Protein, deoxyribonuclease I Protein, deoxyribonuclease I L homeolog Protein, Deoxyribonuclease I Protein, endA Protein, LOC397802 Protein, DNASE1 Protein, Dnase1 Protein, dnase1.L Protein, dnase1 Protein, CpipJ_CPIJ011184 Protein, CpipJ_CPIJ011185 Protein, CpipJ_CPIJ011186 Protein, CpipJ_CPIJ011187 Protein, CpipJ_CPIJ011190 Protein, CpipJ_CPIJ017803 Protein, Desal_1409 Protein, MPET_RS05255 Protein, METEV_RS02275 Protein, Plabr_0631 Protein

Background:

Abbreviation: DNase-I

Target Synonym: DNL1,DRNI,DNL1,Deoxyribonuclease-1,DNase I

Background: DNase1, also known as deoxyribonuclease I and DNL1, is a member of the DNase family. DNaseI is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. DNaseI binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. Mutations in DNase1 gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. DNase1 is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.

Molecular Weight:

Calculated MW: 25.96 kDa

Observed MW: 31 kDa

UniProt: P24855