PNLIP Protein (AA 245-465) (His tag)
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- Target See all PNLIP Proteins
- PNLIP (Pancreatic Lipase (PNLIP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 245-465
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PNLIP protein is labelled with His tag.
- Sequence
- Pro 245-Cys 465
- Characteristics
- A DNA sequence encoding the Human PL protein (P16233) (Pro245-Cys465) was expressed with a N-His .
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product PNLIP Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- PNLIP (Pancreatic Lipase (PNLIP))
- Alternative Name
- Pancreatic lipase (PNLIP Products)
- Synonyms
- PL Protein, PNLIPD Protein, PTL Protein, LIPASE Protein, PANLI Protein, 1810007A24Rik Protein, PNLIP Protein, PNLIPRP2 Protein, pancreatic lipase Protein, PNLIP Protein, Pnlip Protein, pnlip Protein
- Background
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Abbreviation: Pancreatic lipase,PL
Target Synonym: Pancreatic triacylglycerol lipase,PL,PTL,Pancreatic lipase,PNLIP
Background: PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.
- Molecular Weight
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Calculated MW: 24.2 kDa
Observed MW: 30 kDa
- UniProt
- P16233
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