ROCK2 Protein (AA 904-1140) (His tag)

Catalog No. ABIN7505071

Product Details

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Protein Type: Recombinant

Protein Characteristics: AA 904-1140

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ROCK2 protein is labelled with His tag.

Sequence: Leu 904-Gly 1140

Characteristics: A DNA sequence encoding the Human Rock-2 protein (O75116) (Leu 904-Gly 1140) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: Rock-2 (ROCK2 Products)

Synonyms: rocka-a Protein, xROK Protein, rock2 Protein, rok2 Protein, ROCK2 Protein, B230113H15Rik Protein, ROKalpha Protein, Rho-kinase Protein, Rock-II Protein, Rock2m Protein, mKIAA0619 Protein, ROCK-II Protein, ROK Protein, Rho-associated, coiled-coil containing protein kinase 2 L homeolog Protein, rho-associated, coiled-coil containing protein kinase 2a Protein, Rho associated coiled-coil containing protein kinase 2 Protein, Rho-associated, coiled-coil containing protein kinase 2 Protein, Rho-associated coiled-coil containing protein kinase 2 Protein, rock2.L Protein, rock2a Protein, ROCK2 Protein, Rock2 Protein

Background:

Abbreviation: Rock-2

Target Synonym: Rho kinase 2,Rho-associated,Rho-associated protein kinase 2,ROCK 2,Rock II,Rock2,ROCK2,Rock2m,ROK alpha,ROKalpha

Background: ROCK2/II (Rho-associated protein kinase 2, also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92?354), a coiled-coil region (aa 429?1131) and a zinc-finger region (aa 1260?1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids.

Molecular Weight:

Calculated MW: 25.96 kDa

Observed MW: 35 kDa

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation