ADA Protein (AA 11-280) (His tag)

Catalog No. ABIN7505066

Product Details

Target: ADA (Adenosine Deaminase (ADA))

Protein Type: Recombinant

Protein Characteristics: AA 11-280

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ADA protein is labelled with His tag.

Sequence: Lys 11-Val 280

Characteristics: A DNA sequence encoding the Human ADA protein (P00813) (Lys 11-Val 280) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: ADA (ADA Products)

Synonyms: ADA-like Protein, xada Protein, ADA Protein, CG11994 Protein, Dmel\\CG11994 Protein, DrosADA Protein, dADA Protein, zgc:92028 Protein, adenosine deaminase Protein, adenosine deaminase S homeolog Protein, Adenosine deaminase Protein, ADA Protein, Ada Protein, ada.S Protein, ada Protein

Background:

Abbreviation: ADA

Target Synonym: ADA,ADA1,adenine deaminase,Adenosine aminohydrolase,Adenosine Deaminase,EC 3.5.4.4

Background: Adenosine Deaminase (ADA, adenosine aminohydrolase) is one of the key enzymes of purine nucleotide catabolism. It catalyses the hydrolytic deamination of adenosine and deoxy?adenosine to inosine and deoxyinosine. ADA is expressed in virtually all tissues and is expressed at high levels in T-lymphocytes. Adenosine Deaminase deficiency can cause a form of SCID (severe combined immunodeficiency) and lymphopenia in both B- and T-cell lineages ADA can be used as a sensitive diagnostic marker for tuberculous pleuritis. Although it is primarily a cytosolic enzyme, ADA is known to be a positive regulator of T-cell co?activation due to its binding to CD26 at the cell surface. The interaction of ADA with CD26 regulates lymphocyte-epithelial cell adhesion.

Molecular Weight:

Calculated MW: 29.59 kDa

Observed MW: 32 kDa

UniProt: P00813

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process