NADPH Oxidase 4 Protein (NOX4) (AA 220-392) (His tag)

Catalog No. ABIN7505064

Product Details

Target: NADPH Oxidase 4 (NOX4)

Protein Type: Recombinant

Protein Characteristics: AA 220-392

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NADPH Oxidase 4 protein is labelled with His tag.

Sequence: Asp 220-Asp 392

Characteristics: A DNA sequence encoding the Human NOX4 protein (Q9NPH5) (Asp 220-Asp 392) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4 (NOX4 Products)

Synonyms: KOX Protein, KOX-1 Protein, RENOX Protein, AI648021 Protein, nox4 Protein, NOX4 Protein, NADPH oxidase 4 Protein, NOX4 Protein, Nox4 Protein, nox4 Protein, PTRG_09562 Protein

Background:

Abbreviation: NOX4

Target Synonym: KOX 1,KOX,Kox-1,KOX1,NADPH,NADPH oxidase Nox4,NOX4,Renal NAD(P)H oxidase,Renal NAD(P)H-oxidase,RENOX

Background: NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases. NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling. NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin. Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress. Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified. Over aa 450-578, human NOX4 has 92 % sequence identity with mouse NOX4, and 91 % identity with rat NOX4.

Molecular Weight:

Calculated MW: 18.92 kDa

Observed MW: 18 kDa

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration