PIBF1 Protein (AA 13-203) (His tag)

Catalog No. ABIN7505055

Product Details

Target: PIBF1 (Progesterone Immunomodulatory Binding Factor 1 (PIBF1))

Protein Type: Recombinant

Protein Characteristics: AA 13-203

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PIBF1 protein is labelled with His tag.

Sequence: Asn 13-Val 203

Characteristics: A DNA sequence encoding the Human PIBF protein (Q8WXW3) (Asn 13-Val 203) was expressed with a N-His tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PIBF1 (Progesterone Immunomodulatory Binding Factor 1 (PIBF1))

Alternative Name: PIBF (PIBF1 Products)

Synonyms: D14Ertd581e Protein, 1700017E21Rik Protein, 4930513H15Rik Protein, 4933438D16Rik Protein, 4933439E17Rik Protein, RGD1305077 Protein, PIBF Protein, C13orf24 Protein, zgc:101063 Protein, MGC80410 Protein, CEP90 Protein, RP11-505F3.1 Protein, progesterone immunomodulatory binding factor 1 Protein, progesterone immunomodulatory binding factor 1 S homeolog Protein, Pibf1 Protein, PIBF1 Protein, pibf1 Protein, pibf1.S Protein

Background:

Abbreviation: PIBF

Target Synonym: C13orf24,KIAA1008,PIBF 1,PIBF,PIBF1,PIBF1,Progesterone immunomodulatory binding factor 1,Progesterone induced blocking factor 1

Background: PIBF-1 (Progesterone induced blocking factor 1) is a 90-92 kDa progesterone-inducible molecule initially isolated from the lymphocytes of pregnant women. It is produced by lymphocytes plus villous trophoblast cells, and modulates the activity of cytotoxic NK cells. Human PIBF-1 is 757 amino acids (aa) in length. It contains a possible signal sequence (aa 1-26), two Leu-zippers (aa 311-323 and 643-664), two NLSs (aa 282-285 and 567-573), a bZIP sequence (aa 574-614), and an ER retention motif (aa 752-757). PIBF-1 is found in the nucleus, cytoplasm, and circulation, the result of multiple splice variants. SDS-page shows 90 kDa, 80-83 kDa, 48-52 kDa, 34-35 kDa, and 10-12 kDa isoforms. The 90 kDa form is nuclear and full-length, while the 34-35 kDa form is secreted and likely represents aa 1-223 spliced to aa 683-757. There is a 50-55 kDa form that is apparently intracellular, possibly bioactive and likely represents the N-terminus of the molecule. Over aa 19?419, human PIBF-1 shares 88 % aa identity with mouse PIBF-1.

Molecular Weight:

Calculated MW: 20.9 kDa

Observed MW: 28 kDa

UniProt: Q8WXW3