NEFM Protein (AA 2-358) (GST tag)
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- Target See all NEFM Proteins
- NEFM (Neurofilament Triplet M Protein (NEFM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-358
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NEFM protein is labelled with GST tag.
- Sequence
- Ser 2-Ser 358
- Characteristics
- A DNA sequence encoding the Human NEF3 protein (P07197) (Ser2-Ser358) was expressed with a GST.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product NEFM Protein
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Neurofilament Triplet M Protein (NEFM) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)
NEFM Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- NEFM (Neurofilament Triplet M Protein (NEFM))
- Alternative Name
- NEF3 (NEFM Products)
- Synonyms
- NEF3 Protein, NF-M Protein, NFM Protein, Nef3 Protein, Nfm Protein, NF160 Protein, NF165 Protein, nef-m Protein, nef3 Protein, nefm Protein, nf-m Protein, zgc:112359 Protein, nefm-a Protein, nefm-b Protein, neurofilament medium Protein, neurofilament triplet M protein Protein, neurofilament, medium polypeptide Protein, neurofilament medium polypeptide Protein, neurofilament medium L homeolog Protein, neurofilament, medium polypeptide a Protein, neurofilament medium S homeolog Protein, NEFM Protein, Nefm Protein, TVAG_582010 Protein, TVAG_379630 Protein, nefm.L Protein, nefma Protein, nefm.S Protein
- Background
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Abbreviation: NEF3
Target Synonym: Neurofilament medium polypeptide,NF-M,160 kDa neurofilament protein,Neurofilament 3,Neurofilament triplet M protein,NEFM,NEF3,NFM
Background: Human NF-M (neurofilament-medium length) is a 145?150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2?358, which includes most of the rod region, shares ~98 % aa sequence identity with corresponding region of mouse, rat and canine NF-M.
- Molecular Weight
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Calculated MW: 64.16 kDa
Observed MW: 60 kDa
- UniProt
- P07197
- Pathways
- Brown Fat Cell Differentiation
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