CDK4 Protein (AA 2-303) (His tag)

Catalog No. ABIN7504963

Product Details

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Protein Type: Recombinant

Protein Characteristics: AA 2-303

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CDK4 protein is labelled with His tag.

Application: Immunogen (Imm)

Sequence: Ala2-Glu303

Characteristics: A DNA sequence encoding the Human CDK4 (P11802-1) (Ala2-Glu303) was expressed with a polyhistidine tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

used as Immunogen for (ABIN6999859)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4 (CDK4 Products)

Synonyms: zgc:153726 Protein, CMM3 Protein, PSK-J3 Protein, Crk3 Protein, 8-6 Protein, CDK4 Protein, CDK4/6 Protein, CG5072 Protein, Cdk4/6 Protein, DmCdk4 Protein, Dmel\\CG5072 Protein, Pk53C Protein, Pk?7 Protein, cdk Protein, cdk4 Protein, cdk4/6 Protein, dCdk4 Protein, l(2)05428 Protein, l(2)0671 Protein, l(2)k06503 Protein, l(2)s4639 Protein, l(2)sh0671 Protein, xcdk4 Protein, cyclin-dependent kinase 4 Protein, cyclin dependent kinase 4 Protein, Cyclin-dependent kinase 4 Protein, cyclin-dependent kinase 4 L homeolog Protein, cdk4 Protein, CDK4 Protein, Cdk4 Protein, cdk4.L Protein

Background:

Abbreviation: CDK4

Target Synonym: Cdk 4,cdk4,CDK4 protein,CDK4,Cell division kinase 4,Cell division protein kinase 4,CMM 3,CMM3,Crk3,Cyclin dependent kinase 4,Cyclin-dependent kinase 4,Melanoma cutaneous malignant 3,MGC14458,p34 cdk4,PSK J3,PSK-J3

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Molecular Weight:

Calculated MW: 33.5 kDa

Observed MW: 35.8 kDa

UniProt: P11802-1

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size