CDK2 Protein (AA 1-298) (His tag)
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- Target See all CDK2 Proteins
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-298
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CDK2 protein is labelled with His tag.
- Sequence
- Met 1-Leu298
- Characteristics
- Recombinant Human Cyclin-Dependent Kinase 2 is produced by our E.coli expression system and the target gene encoding Met1-Leu298 is expressed with a 6His tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- <1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product CDK2 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- Alternative Name
- CDK2 (CDK2 Products)
- Synonyms
- p33(CDK2) Protein, A630093N05Rik Protein, wu:fa10c02 Protein, zgc:56598 Protein, zgc:77684 Protein, eg1 Protein, CDKN2 Protein, cdk2L Protein, cyclin dependent kinase 2 Protein, cyclin-dependent kinase 2 Protein, cyclin-dependent kinase 2 S homeolog Protein, CDK2 Protein, Cdk2 Protein, cdk2 Protein, cdk2.S Protein
- Background
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Abbreviation: CDK2
Target Synonym: Cyclin-Dependent Kinase 2,Cell Division Protein Kinase 2,p33 Protein Kinase,CDK2,CDKN2
Background: Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.
- Molecular Weight
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Calculated MW: 36.1 kDa
Observed MW: 34 kDa
- UniProt
- P24941
- Pathways
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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