SKP1 Protein (AA 2-163) (Biotin)
-
- Target See all SKP1 Proteins
- SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-163
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SKP1 protein is labelled with Biotin.
- Purpose
- Biotinylated Human SKP1 Protein
- Sequence
- Pro2-Lys163
- Characteristics
- Recombinant Biotinylated Human SKP1 Protein is expressed from HEK293 His tag at the N-terminus and Avi tag at the C-terminusIt contains Pro2-Lys163.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product SKP1 Protein
-
S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-160) protein (GST tag)
SKP1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-160) proteinSKP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
S-Phase Kinase-Associated Protein 1 (SKP1) (AA 2-163) protein (GST tag)SKP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
S-Phase Kinase-Associated Protein 1 (SKP1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SKP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))
- Alternative Name
- SKP1 (SKP1 Products)
- Background
- The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.
- Molecular Weight
- 21.43 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
- Pathways
- Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases
-
